N-colour separation methods for accurate reproduction of spot colours

In packaging, spot colours are used to print key information like brand logos and elements for which the colour accuracy is critical. The present study investigates methods to aid the accurate reproduction of these spot colours with the n-colour printing process. Typical n-colour printing systems consist of supplementary inks in addition to the usual CMYK inks. Adding these inks to the traditional CMYK set increases the attainable colour gamut, but the added complexity creates several challenges in generating suitable colour separations for rendering colour images. In this project, the n-colour separation is achieved by the use of additional sectors for intermediate inks. Each sector contains four inks with the achromatic ink (black) common to all sectors. This allows the extension of the principles of the CMYK printing process to these additional sectors. The methods developed in this study can be generalised to any number of inks. The project explores various aspects of the n-colour printing process including the forward characterisation methods, gamut prediction of the n-colour process and the inverse characterisation to calculate the n-colour separation for target spot colours. The scope of the study covers different printing technologies including lithographic offset, flexographic, thermal sublimation and inkjet printing. A new method is proposed to characterise the printing devices. This method, the spot colour overprint (SCOP) model, was evaluated for the n-colour printing process with different printing technologies. In addition, a set of real-world spot colours were converted to n-colour separations and printed with the 7-colour printing process to evaluate against the original spot colours. The results show that the proposed methods can be effectively used to replace the spot coloured inks with the n-colour printing process. This can save significant material, time and costs in the packaging industry

A CONCEPTUAL STUDY ON PANCHAKARMA APPROACH IN THE MANAGEMENT OF ANKYLOSING SPONDYLITIS

Ankylosing spondylitis is characterized by a chronic inflammatory arthritis predominantly affecting the sacro-iliac joints and spine, which can progress to bony fusion of the spine. The onset is typically between the ages of 20-30, with male preponderance of about 3:1. The main symptoms like musculoskeletal pain, stiffness and decreased range of movements in the spine. Modern science has very limited options to treat Ankylosing spondylitis. So, the necessity of management through Ayurveda is very much essential. Various disease entities like Amavata, Gambhira Vatarakta, Asthimajjagata vata can be considered under the spectrum of Ankylosing Spondylitis. By understanding the symptoms of Ankylosing Spondylitis, the pathology pertaining to the Ankylosing Spondylitis can be considered under the Sama and Nirama avastha. A thorough differentiation of Sama and Nirama avastha of Ankylosing spondylitis (Gambhira Vatarakta) has to be done based on the same, Panchakarma procedures are to be adopted. Among various modalities of treatments, Panchakarma can be the better option to treat this condition. Hence, a conceptual study was taken up to develop an approach through Panchakarma modalities in the management of Ankylosing Spondylitis

ROLE OF PANCHAKARMA IN PARKINSONS DISEASE - A CASE STUDY

Parkinsons disease is a degenerative disorder of the central nervous system. The motor symptoms of Parkinsons disease result from the death of dopamine-generating cells in the substantia nigra, a region of the midbrain; the cause of this cell death is unknown. Early in the course of disease symptoms include (1) shaking (tremor) (2)  rigidity (3) slowness of movement  (bradykinesia) and (4) difficulty with walking and gait (postural instability). Later, cognitive and behavioral problems may arise, with dementia commonly occurring in the advanced stages of the disease. Other symptoms include sensory, sleep and emotional problems. In Ayurveda, most of the diseases of the Vata are essentially the conditions of degenerative diseases of the nervous system. Kampa vata is one condition which is caused by imbalance of Vata. The treatment of Kampavata consists of both internal and external administration of drugs in different forms aimed to reverse the Vata imbalance. Here a case of 75 years male patient presented with tremors in both hands and postural instability and the pathology of which is to be identified with Dhatukshayaja Vata Vyadhi which is provisionally diagnosed as Kampavata and its line of treatment was adopted based on Nirupasthambita Vata Vyadhi Chikitsa. The present case reveals certain set of Panchakarma modalities of treatments showing significant improvement in the symptoms of Parkinsons disease without causing any side effects

Optical polarimetry of the blazar CGRaBS~ J0211+1051 from MIRO

We report the detection of high polarization in the first detailed optical linear polarization measurements on the BL Lac object CGRaBS J0211+1051, which flared in $\gamma$-rays on 2011 January 23 as reported by Fermi. The observations were made during 2011 January 30 - February 3 using photo-polarimeter mounted at the 1.2m telescope of Mt Abu InfraRed Observatory(MIRO). The CGRaBS J0211+1051 was detected to have $\sim21.05\pm 0.41$ degree of polarization (DP) with steady position angle (PA) at 43$^\circ$ on 2011 January 30. During Jan 31 and Feb 1, while polarization shows some variation, position angle remains steady for the night. Several olarization flashes occurred during February 2 and 3 resulting in changes in the DP by more than 4% at short time scales ($\sim$ 17 to 45 mins). A mild increase in the linear polarization with frequency is noticed during the nights of February 2 & 3. The source exhibited significant inter-night variations in the degree of polarization (changed by about 2 to 9%) and position angle (changed by 2 to 22$^\circ$) during the five nights of observations. The intra-night activity shown by the source appears to be related to turbulence in the relativistic jet. Sudden change in the PA accompanied by a rise in the DP could be indicative of the fresh injection of electrons in the jet. The detection of high and variable degree of polarization categorizes the source as low energy peaked blazar.Comment: 14-pages including 3-figure

What makes the pregnant women revisit public hospitals for research? Participant engagement and retention trial in a public hospital (PERTH): an RCT protocol.

BACKGROUND: Cohort studies have public health importance as they effectively provide evidence on determinants of health from a life course perspective. Researchers often confront the poor follow-up rates as a major challenge in the successful conduct of cohort studies. We are currently recruiting in a birth cohort study, titled as "Maternal Antecedents of Adiposity and Studying the Transgenerational role of Hyperglycemia and Insulin" (MAASTHI) in a public hospital; with the aim of assessing maternal glycemic levels on the risk of adverse fetal outcomes. Nested within the ongoing cohort, the proposed trial aims to evaluate the effectiveness of two interventions in improving the follow-up in the cohort study in a public hospital. METHODS: A randomized trial of 795 pregnant women, with 265 women each in three arms observed through pregnancy, until their baby is 14 weeks old. The comparator group receives a standard leaflet, with details on the importance of glucose testing and regular follow up in pregnancy. Intervention arm-1 will receive the standard leaflet plus individualized messages, through an Interactive Voice Response (IVR) system; a type of computer-linked telephone intervention system to remind the participants about the lab test and follow-up dates. Intervention arm- 2 will have the opportunity to attend Mother and Baby Affairs (MBA) workshops, which will provide information on Gestational Diabetes Mellitus (GDM) screening and management to pregnant women and personalized counselling services. The outcome of interest is the difference in the proportion of participants completing follow-up at different points in time, among three arms. DISCUSSION: Between the two interventions (IVR and MBA), the study results would uncover the contextually specific, timely intervention, which can increase the proportion of pregnant women followed up in public hospitals. If effective, this study will provide information on an effective intervention, useful in ensuring the success of longitudinal follow-up in the public hospitals. TRIAL REGISTRATION: NCT03088501 , Date Registered: 16/03/2017

Maternal antecedents of adiposity and studying the transgenerational role of hyperglycemia and insulin (MAASTHI): a prospective cohort study : Protocol of birth cohort at Bangalore, India.

BACKGROUND: India is experiencing an epidemic of obesity-hyperglycaemia, which coincides with child bearing age for women. The epidemic can be sustained and augmented through transgenerational transmission of adiposity and glucose intolerance in women. This presents an opportunity for exploring a clear strategy for the control of this epidemic in India. We conducted a study between November 2013 and May 2015 to inform the design of a large pregnancy cohort study. Based on the findings of this pilot, we developed the protocol for the proposed birth cohort of 5000 women, the recruitment for which will start in April 2016. The protocol of the study documents the processes which aim at advancing the available knowledge, linking several steps in the evolution of obesity led hyperglycemia. METHODS: Maternal Antecedents of Adiposity and Studying the Transgenerational role of Hyperglycemia and Insulin (MAASTHI) is a cohort study in the public health facilities in Bangalore, India. The objective of MAASTHI is to prospectively assess the effects of glucose levels in pregnancy on the risk of adverse infant outcomes, especially in predicting the possible risk markers of later chronic diseases. The primary objective of the proposed study is to investigate the effect of glucose levels in pregnancy on skinfold thickness (adiposity) in infancy as a marker of future obesity and diabetes in offspring. The secondary objective is to assess the association between psychosocial environment of mothers and adverse neonatal outcomes including adiposity. The study aims to recruit 5000 pregnant women and follow them and their offspring for a period of 4 years. The institutional review board at The Indian Institute of Public Health (IIPH)-H, Bangalore, Public Health Foundation of India has approved the protocol. All participants are required to provide written informed consent. DISCUSSION: The findings from this study may help to address important questions on screening and management of high blood sugar in pregnancy. It may provide critical information on the specific determinants driving the underweight-obesity-T2DM epidemic in India. The study can inform the policy regarding the potential impact of screening and management protocols in public healthcare facilities. The public health implications include prioritising issues of maternal glycemic control and weight management and better understanding of the lifecourse determinants in the development of T2DM

BioBuilder as a database development and functional annotation platform for proteins

BACKGROUND: The explosion in biological information creates the need for databases that are easy to develop, easy to maintain and can be easily manipulated by annotators who are most likely to be biologists. However, deployment of scalable and extensible databases is not an easy task and generally requires substantial expertise in database development. RESULTS: BioBuilder is a Zope-based software tool that was developed to facilitate intuitive creation of protein databases. Protein data can be entered and annotated through web forms along with the flexibility to add customized annotation features to protein entries. A built-in review system permits a global team of scientists to coordinate their annotation efforts. We have already used BioBuilder to develop Human Protein Reference Database , a comprehensive annotated repository of the human proteome. The data can be exported in the extensible markup language (XML) format, which is rapidly becoming as the standard format for data exchange. CONCLUSIONS: As the proteomic data for several organisms begins to accumulate, BioBuilder will prove to be an invaluable platform for functional annotation and development of customizable protein centric databases. BioBuilder is open source and is available under the terms of LGPL

Evaluation of genetic diversity in Magnaporthe grisea populations adapted to finger millet using simple sequence repeats (SSRs) markers

Finger millet blast caused by Magnaporthe grisea (anamorph: Pyricularia grisea) is a great threat to finger millet production worldwide. Genetic diversity and population structure of 72 M. grisea isolates collected from finger millet (56), foxtail millet (6), pearl millet (7) and rice (3) from major crop growing areas in India was studied using 24 SSR markers. None of the SSRs detected polymorphism in the M. grisea isolates from pearl millet. Seventeen SSR markers were polymorphic in the 65 non pearl millet isolates and detected 105 alleles, of which one was rare, 83 common, 9 frequent and 12 most frequent. A model-based population structure analysis of the genomic data identified two distinct populations with varying levels of ancestral admixtures among the 65 M. grisea isolates. Analysis of molecular variance (AMOVA) indicated that 52% of the total variation among the isolates used in this study was due to differences between the pathogen populations adapted to different hosts, 42% was due to differences in the isolates from the same host, and the remaining 6% due to heterozygosity within isolates. High genetic variability present in M. grisea isolates calls for the continuous monitoring of M. grisea populations anticipating blast resistance breakdown in finger millet cultivars grown in India

Congenital myasthenic syndrome with mild intellectual disability caused by a recurrent SLC25A1 variant

Abstract: Congenital myasthenic syndromes (CMS) are a clinically and genetically heterogeneous group of disorders caused by mutations which lead to impaired neuromuscular transmission. SLC25A1 encodes a mitochondrial citrate carrier, associated mainly with the severe neurometabolic disease combined D-2- and L-2-hydroxyglutaric aciduria (D/L-2-HGA). We previously reported a single family with a homozygous missense variant in SLC25A1 with a phenotype restricted to relatively mild CMS with intellectual disability, but to date no additional cases of this CMS subtype had been reported. Here, we performed whole exome sequencing (WES) in three additional and unrelated families presenting with CMS and mild intellectual disability to identify the underlying causative gene. The WES analysis revealed the presence of a homozygous c.740G>A; p.(Arg247Gln) missense SLC25A1 variant, the same SLC25A1 variant as identified in the original family with this phenotype. Electron microscopy of muscle from two cases revealed enlarged and accumulated mitochondria. Haplotype analysis performed in two unrelated families suggested that this variant is a result of recurrent mutation and not a founder effect. This suggests that p.(Arg247Gln) is associated with a relatively mild CMS phenotype with subtle mitochondrial abnormalities, while other variants in this gene cause more severe neurometabolic disease. In conclusion, the p.(Arg247Gln) SLC25A1 variant should be considered in patients presenting with a presynaptic CMS phenotype, particularly with accompanying intellectual disability